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2a egfppgk puro cassette  (Addgene inc)


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    Structured Review

    Addgene inc 2a egfppgk puro cassette
    2a Egfppgk Puro Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2a+egfppgk+puro+cassette/pDONR221_SLC25A12+(Plasmid+%23131938)/pm28459451-410-6-8
    Average 90 stars, based on 1 article reviews
    2a egfppgk puro cassette - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Construct:

    Article Title: Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
    Article Snippet: Generation of ATOH1-2A-eGFP reporter cell line. gRNAs (5′-TCGGAT GAGGCAAGTTAGGA-3′ and 5′-GTCACTGTAATGGGAATGGG-3′, offset = 0 bp) targeting the stop codon region of ATOH1 were cloned into pSpCas9n(BB) vectors which express Cas9n under the control of the CBh promoter (Addgene #48873)38. .. To construct the donor vector, a 2A-eGFPPGK-Puro cassette (Addgene #31938)39 flanked by two 1-kb homology arms PCR amplified from extracted WA25 hESC genomic DNA were cloned into a pUC19 backbone. .. The two gRNA vectors and the donor vector, as well as a vector expressing Cas9n under the control of the CMV promoter (Addgene #41816)40 were transfected into WA25 hESCs with 4D Nucleofector (Lonza) using the P3 Primary Cell 4D-Nucleofector X kit and Program CB-150.

    Plasmid Preparation:

    Article Title: Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
    Article Snippet: Generation of ATOH1-2A-eGFP reporter cell line. gRNAs (5′-TCGGAT GAGGCAAGTTAGGA-3′ and 5′-GTCACTGTAATGGGAATGGG-3′, offset = 0 bp) targeting the stop codon region of ATOH1 were cloned into pSpCas9n(BB) vectors which express Cas9n under the control of the CBh promoter (Addgene #48873)38. .. To construct the donor vector, a 2A-eGFPPGK-Puro cassette (Addgene #31938)39 flanked by two 1-kb homology arms PCR amplified from extracted WA25 hESC genomic DNA were cloned into a pUC19 backbone. .. The two gRNA vectors and the donor vector, as well as a vector expressing Cas9n under the control of the CMV promoter (Addgene #41816)40 were transfected into WA25 hESCs with 4D Nucleofector (Lonza) using the P3 Primary Cell 4D-Nucleofector X kit and Program CB-150.

    Polymerase Chain Reaction:

    Article Title: Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
    Article Snippet: Generation of ATOH1-2A-eGFP reporter cell line. gRNAs (5′-TCGGAT GAGGCAAGTTAGGA-3′ and 5′-GTCACTGTAATGGGAATGGG-3′, offset = 0 bp) targeting the stop codon region of ATOH1 were cloned into pSpCas9n(BB) vectors which express Cas9n under the control of the CBh promoter (Addgene #48873)38. .. To construct the donor vector, a 2A-eGFPPGK-Puro cassette (Addgene #31938)39 flanked by two 1-kb homology arms PCR amplified from extracted WA25 hESC genomic DNA were cloned into a pUC19 backbone. .. The two gRNA vectors and the donor vector, as well as a vector expressing Cas9n under the control of the CMV promoter (Addgene #41816)40 were transfected into WA25 hESCs with 4D Nucleofector (Lonza) using the P3 Primary Cell 4D-Nucleofector X kit and Program CB-150.

    Amplification:

    Article Title: Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
    Article Snippet: Generation of ATOH1-2A-eGFP reporter cell line. gRNAs (5′-TCGGAT GAGGCAAGTTAGGA-3′ and 5′-GTCACTGTAATGGGAATGGG-3′, offset = 0 bp) targeting the stop codon region of ATOH1 were cloned into pSpCas9n(BB) vectors which express Cas9n under the control of the CBh promoter (Addgene #48873)38. .. To construct the donor vector, a 2A-eGFPPGK-Puro cassette (Addgene #31938)39 flanked by two 1-kb homology arms PCR amplified from extracted WA25 hESC genomic DNA were cloned into a pUC19 backbone. .. The two gRNA vectors and the donor vector, as well as a vector expressing Cas9n under the control of the CMV promoter (Addgene #41816)40 were transfected into WA25 hESCs with 4D Nucleofector (Lonza) using the P3 Primary Cell 4D-Nucleofector X kit and Program CB-150.

    Clone Assay:

    Article Title: Generation of inner ear organoids containing functional hair cells from human pluripotent stem cells.
    Article Snippet: Generation of ATOH1-2A-eGFP reporter cell line. gRNAs (5′-TCGGAT GAGGCAAGTTAGGA-3′ and 5′-GTCACTGTAATGGGAATGGG-3′, offset = 0 bp) targeting the stop codon region of ATOH1 were cloned into pSpCas9n(BB) vectors which express Cas9n under the control of the CBh promoter (Addgene #48873)38. .. To construct the donor vector, a 2A-eGFPPGK-Puro cassette (Addgene #31938)39 flanked by two 1-kb homology arms PCR amplified from extracted WA25 hESC genomic DNA were cloned into a pUC19 backbone. .. The two gRNA vectors and the donor vector, as well as a vector expressing Cas9n under the control of the CMV promoter (Addgene #41816)40 were transfected into WA25 hESCs with 4D Nucleofector (Lonza) using the P3 Primary Cell 4D-Nucleofector X kit and Program CB-150.



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    2a egfppgk puro cassette  (Addgene inc)
    90
    Addgene inc 2a egfppgk puro cassette
    2a Egfppgk Puro Cassette, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/2a+egfppgk+puro+cassette/pDONR221_SLC25A12+(Plasmid+%23131938)/pm28459451-410-6-8
    Average 90 stars, based on 1 article reviews
    2a egfppgk puro cassette - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

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